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PT-141 (Bremelanotide) Half-Life: ~2.7 Hours — Pharmacokinetics & Dosing

Also known as: bremelanotide · Vyleesi · PT141

FDA-Approved· NDA 210557 (Vyleesi) — HSDD in premenopausal women · Half-life from human RCT PMID 26944463

Quick Reference — PT-141 Pharmacokinetics

ParameterValueSource
Elimination Half-Life (SC)~2.7 hoursFDA NDA 210557; Clayton AH et al. PMID 26944463
Tmax (SC)~1 hourFDA NDA 210557
Route(s) of AdministrationSubcutaneous autoinjector (abdomen or thigh)
Plasma Protein Binding~21%FDA NDA 210557
Time to Steady StateN/A — PRN dosing (not daily use)
Accumulation at Steady StateMinimal (PRN use; max 1 dose per 24 h)
Full Clearance (5 half-lives)~13.5 hoursCalculated from NDA 210557 t½
Standard Dosing Frequency1.75 mg SC PRN ≥45 min before activity; max 1 per 24 hFDA NDA 210557
Data QualityHuman RCT — FDA NDA 210557; Clayton AH et al. J Sex Med 2016 PMID 26944463
Reviewed by Halflife Labs Medical Review Team Last reviewed May 2026 Evidence level Human RCT · Methodology

What Is the Half-Life of PT-141 (Bremelanotide)?

PT-141 (bremelanotide, trade name Vyleesi) has an elimination half-life of approximately 2.7 hours after subcutaneous injection, based on FDA NDA 210557 pharmacokinetic data and human RCT evidence.[1][2] With a Tmax of approximately 1 hour, peak plasma concentrations are reached within the first hour, and the compound is substantially cleared from plasma (~97%) within approximately 13.5 hours of dosing. This PK profile supports the approved PRN (as-needed) dosing paradigm.

Bremelanotide is a synthetic cyclic hepta-peptide derived from Melanotan II, approved by the FDA in June 2019 under NDA 210557 for the treatment of hypoactive sexual desire disorder (HSDD) in premenopausal women.[1]

How PT-141's Half-Life Is Measured

The PK data in FDA NDA 210557 characterizes the plasma concentration-time profile after a single 1.75 mg SC dose. The terminal elimination half-life of ~2.7 hours is derived from population PK modeling and single-dose PK studies. The Clayton et al. (2016) Phase 2 dose-finding RCT confirmed the ~2.7-hour half-life at the 1.75 mg dose used in Phase 3 trials.[2]

Plasma Half-Life vs. Biological Effect Duration

PT-141's ~2.7-hour plasma half-life is shorter than its observed clinical effect duration of approximately 6–12 hours for pro-sexual desire effects. Once MC4R signaling cascades are activated in hypothalamic nuclei (medial preoptic area), the downstream neural circuits mediating sexual motivation continue to operate at plasma concentrations below the peak. The clinical instruction to inject ≥45 minutes before activity reflects the Tmax (~1 hour) — onset of peak CNS exposure — not the end of the effect window.[1][3]

How Long Does PT-141 Stay in Your System?

After a single 1.75 mg subcutaneous dose, PT-141 plasma concentrations decline with a t½ of ~2.7 hours. Full clearance occurs within approximately 13.5 hours.

Clearance Timeline — After a Single Dose

Half-Lives ElapsedTime After Dose% Remaining in PlasmaClinical Note
1~2.7 hours50%Within peak effect window (Tmax ~1 h)
2~5.4 hours25%Clinical effects typically ongoing
3~8.1 hours12.5%Effects beginning to taper
4~10.8 hours6.25%Near-complete plasma clearance
5 (clinical clearance threshold)~13.5 hours~3%Pharmacologically negligible; consistent with max 1 per 24 h limit
⚠ PT-141 causes transient blood pressure elevation. Mean systolic BP increases approximately 6 mmHg for up to 12 hours post-dose. Patients with cardiovascular disease should consult a physician before use. Source: FDA NDA 210557 prescribing information.

Dosing Implications of PT-141's Half-Life

Why As-Needed (PRN) Dosing?

PT-141's ~2.7-hour half-life and ~13.5-hour full clearance period are suited to PRN dosing. The compound reaches peak plasma concentration within ~1 hour, produces effects lasting approximately 6–12 hours, and is substantially cleared within 13–14 hours — compatible with situational pre-activity use. Daily dosing is not required because the MC4R mechanism does not require tonic receptor occupancy; acute activation is sufficient to produce the desired neural effect.[1]

Missed Dose — Effect on Blood Levels

PT-141 is PRN rather than scheduled, so the concept of a missed dose does not apply pharmacokinetically. There is no trough-maintenance requirement and no accumulation effect under the approved once-per-24-hour maximum. Each dose is pharmacologically independent.

PT-141 vs. Flibanserin — Half-Life and Mechanism Comparison

CompoundHalf-LifeDosingMechanismStatus
PT-141 (Bremelanotide / Vyleesi)~2.7 h (SC)PRN ≥45 min before activityMC4R agonist (CNS hypothalamus)FDA-approved NDA 210557
Flibanserin (Addyi)~11 h (oral)100 mg oral once daily at bedtime5-HT1A agonist / 5-HT2A antagonistFDA-approved NDA 022526
Oxytocin (intranasal)~30 min (intranasal)PRN intranasal before activityOxytocin receptor (CNS)Not FDA-approved in US for sexual function

Pharmacokinetics by Route of Administration

RouteHalf-LifeNotes
Subcutaneous (abdomen, thigh)~2.7 hoursOnly approved route; NDA 210557 data. Well-absorbed.
Intranasal (historical)Shorter than SC (estimated); no current clinical dataEarlier development candidate; not the approved route or current formulation.
IntravenousShorter (no published clinical data)Not a clinical or approved route.
OralNot viableProteolytic GI degradation of cyclic peptide; not a viable route.

Detection Window

Standard Drug Test Panels

Bremelanotide (Vyleesi) is FDA-approved and not included in standard WADA or workplace drug screening immunoassay panels. It is not classified as a substance of abuse and is not screened by routine panels.

Specialized Testing (LC-MS/MS)

Specialized LC-MS/MS can detect bremelanotide and its primary metabolite (des-cyclo tetrapeptide fragment) in plasma and urine. Given the ~2.7-hour plasma half-life and full clearance within ~13.5 hours, urine detection using specialized methods would be expected to be limited to approximately 12–24 hours after the last dose. No published forensic urinary detection window study specific to standard panel immunoassay for Vyleesi has been identified.

Mechanism — Why Does PT-141 Have This Half-Life?

Bremelanotide is a synthetic cyclic hepta-peptide (Ac-Nle-cyclo[Asp-His-d-Phe-Arg-Trp-Lys]-OH, molecular weight ~1,025 Da). The cyclic ring structure provides substantially greater proteolytic resistance than linear peptides of similar size — the ring conformation limits protease access to peptide bonds.[1] This metabolic stability extends the half-life from the seconds-to-minutes of native α-MSH to approximately 2.7 hours.

Despite the cyclic structure, bremelanotide is still cleared by hepatic metabolism and renal excretion of metabolite fragments. Plasma protein binding is approximately 21% — low, meaning the unbound fraction available for clearance is high. The absence of albumin-binding side chains or FcRn-recycling mechanisms limits the half-life to the ~2.7-hour range rather than extending it to days.[1]

Melanocortin receptor selectivity: bremelanotide binds MC1R, MC3R, MC4R, and MC5R. The pro-sexual desire effect is attributed primarily to MC4R agonism in the medial hypothalamus. MC1R activation contributes to transient skin flushing; off-target MC3R/MC5R activation contributes to nausea — the most common adverse effect in clinical trials.[3]

Track Your PT-141 Protocol

Log PT-141 dose and injection time. Halflife models your plasma concentration curve based on the ~2.7-hour half-life, showing exactly when you are in the peak-effect window. On-device, no account required.

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Frequently Asked Questions

What is the half-life of PT-141 (bremelanotide)?
PT-141 (bremelanotide/Vyleesi) has an elimination half-life of approximately 2.7 hours after subcutaneous injection. Source: FDA NDA 210557; Clayton AH et al. J Sex Med. 2016;12(3):325–337. PMID 26944463. With Tmax ~1 hour and t½ ~2.7 hours, plasma is substantially cleared (~97%) within ~13.5 hours, consistent with the PRN dosing paradigm and max-once-per-24-hours restriction.
How long does PT-141 stay in your system after stopping?
After a single 1.75 mg SC dose, ~97% of PT-141 is cleared from plasma within 5 half-lives (~13.5 hours). Sexual-desire effects typically last 6–12 hours — longer than the plasma half-life — due to sustained MC4R signaling in hypothalamic circuits that outlasts peak plasma concentration. Source: FDA NDA 210557.
How does PT-141's half-life affect when to take it?
Inject at least 45 minutes before anticipated sexual activity per FDA labeling. Tmax is ~1 hour; the 45-minute window allows plasma to approach peak when CNS MC4R activation is needed. Effects typically persist 6–12 hours. Maximum one dose per 24 hours. Source: FDA NDA 210557.
Can PT-141 be detected on a drug test?
FDA-approved medication; not included in standard WADA or workplace immunoassay panels. Specialized LC-MS/MS can detect bremelanotide, but detection windows are limited (~12–24 hours given the ~2.7-hour plasma t½). FDA-approved medications are disclosed separately from illicit substance panels.
What is the difference between PT-141's plasma half-life and how long its effects last?
Plasma half-life ~2.7 hours; clinical effects ~6–12 hours. Once MC4R-dependent neural circuits in the hypothalamus are activated, they continue operating at sub-peak plasma levels. This PK/PD dissociation is why the effect duration outlasts the plasma half-life by 2–3×. Source: FDA NDA 210557; Simon JA et al. PMID 31599845.
How does PT-141 compare to flibanserin (Addyi) in terms of half-life and mechanism?
PT-141 (Vyleesi): t½ ~2.7 h, PRN SC injection, MC4R agonist. Flibanserin (Addyi): t½ ~11 h, once-daily oral at bedtime, 5-HT1A agonist / 5-HT2A antagonist. Both FDA-approved for HSDD in premenopausal women. PT-141 is situational; flibanserin requires daily chronic use. Alcohol is contraindicated with flibanserin (serious hypotension risk) but not with PT-141.
What is the approved dose of PT-141 and why is it limited to one dose per 24 hours?
1.75 mg SC, ≥45 min before activity, max once per 24 hours. The 24-hour restriction reflects a blood pressure safety consideration — bremelanotide transiently increases mean systolic BP ~6 mmHg for up to 12 hours post-dose — not a pharmacokinetic accumulation concern. Source: FDA NDA 210557.
Why does PT-141 cause nausea, and how long does nausea last?
Nausea occurs in ~40% of patients due to off-target MC3R and MC4R activation in brainstem emetic regions. Onset correlates with Tmax (~1 hour) and typically resolves within 2–4 hours, consistent with the ~2.7-hour half-life. Pre-treatment with an antiemetic may help in susceptible individuals. Source: FDA NDA 210557; Simon JA et al. PMID 31599845.

Related Compounds

References

  1. FDA. NDA 210557 — Vyleesi (bremelanotide) Prescribing Information. Palatin Technologies / AMAG Pharmaceuticals. Approved June 2019. FDA accessdata.fda.gov
  2. Clayton AH, et al. Bremelanotide for Female Sexual Dysfunctions in Premenopausal Women: A Randomized, Placebo-Controlled Dose-Finding Trial. Womens Health (Lond). 2016;12(3):325–337. PMID 26944463
  3. Simon JA, et al. Bremelanotide for Female Sexual Interest/Arousal Disorder: Two Randomized Phase 3 Trials. Obstet Gynecol. 2019;134(5):899–908. PMID 31599845
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